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fluorescent dye kit jc-1  (Elabscience Biotechnology)


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    Elabscience Biotechnology fluorescent dye kit jc-1
    Fluorescent Dye Kit Jc 1, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+dye+kit+jc-1/fluorescent+dye+kit+jc+1/pm39956401-80-5-14
    Average 90 stars, based on 1 article reviews
    fluorescent dye kit jc-1 - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: CRISPR/Cas9 based knockout of lncRNA MALAT1 attenuates TGF-β1 induced Smad 2/3 mediated fibrosis during AKI-to-CKD transition.
    Article Snippet: The analysis was performed using JC-1, a fluorescent dye kit, following the manufacturer's protocol (Elabscience).



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    Fig. 8 D-lactate catabolism prevents ESCC CSCs ferroptosis to sustain their stem-like functions. a Cell viability analysis showing the IC50 value of D-lactate in KYSE410 and KYSE450 cells. b MTS measurement for cell viability of KYSE410 and KYSE450 cells treated with 8 mM and 16 mM D-lactate (D) alone or supplementing ZVAD, CQ, FER and NEC simultaneously. c Immunoblotting results suggesting the expression of ferroptosis markers in KYSE410 and KYSE450 cells with 8 mM or 16 mM D-lactate for 72 h. β-actin was used as an internal reference. d Flow cytometry analysis for lipid reactive oxygen species generation in KYSE410 and KYSE450 cells treated with D-lactate (D). e Histogram showing the relative reactive oxygen species production under the action of 8 mM or 16 mM D-lactate in KYSE410 and KYSE450 cells, respectively. f Flow cytometry results indicating the alternative <t>mitochondrial</t> membrane potential induced by 8 mM or 16 mM <t>D-lactate.</t> <t>JC-1</t> monomers and JC-1 aggregates represent low and high MMP, respectively. g Histogram demonstrating relative mitochondrial membrane potential in KYSE410 and KYSE450 cells treated with D-lactate. h Histogram indicating changed Fe2+ concentration induced by D-lactate in KYSE410 and KYSE450 cells. i Histograms suggesting the changes of intracellular total GSH, reduced GSH and GSSG content in KYSE410 and KYSE450 cells treated with D-lactate. j MTS measurement showing the sensitivity of ESCC cells on Erastin treatment after CDK7-YAP-LDHD axis was up- regulated. k Working model of D-lactate catabolism driven by CDK7-YAP-LDHD axis promotes pyruvate generation and helps ESCC cells escape from ferroptosis induced by D-lactate, then support ESCC-CSCs’ properties. Error bars represent mean ± SD (n = 3)
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    Fig. 8 D-lactate catabolism prevents ESCC CSCs ferroptosis to sustain their stem-like functions. a Cell viability analysis showing the IC50 value of D-lactate in KYSE410 and KYSE450 cells. b MTS measurement for cell viability of KYSE410 and KYSE450 cells treated with 8 mM and 16 mM D-lactate (D) alone or supplementing ZVAD, CQ, FER and NEC simultaneously. c Immunoblotting results suggesting the expression of ferroptosis markers in KYSE410 and KYSE450 cells with 8 mM or 16 mM D-lactate for 72 h. β-actin was used as an internal reference. d Flow cytometry analysis for lipid reactive oxygen species generation in KYSE410 and KYSE450 cells treated with D-lactate (D). e Histogram showing the relative reactive oxygen species production under the action of 8 mM or 16 mM D-lactate in KYSE410 and KYSE450 cells, respectively. f Flow cytometry results indicating the alternative <t>mitochondrial</t> membrane potential induced by 8 mM or 16 mM <t>D-lactate.</t> <t>JC-1</t> monomers and JC-1 aggregates represent low and high MMP, respectively. g Histogram demonstrating relative mitochondrial membrane potential in KYSE410 and KYSE450 cells treated with D-lactate. h Histogram indicating changed Fe2+ concentration induced by D-lactate in KYSE410 and KYSE450 cells. i Histograms suggesting the changes of intracellular total GSH, reduced GSH and GSSG content in KYSE410 and KYSE450 cells treated with D-lactate. j MTS measurement showing the sensitivity of ESCC cells on Erastin treatment after CDK7-YAP-LDHD axis was up- regulated. k Working model of D-lactate catabolism driven by CDK7-YAP-LDHD axis promotes pyruvate generation and helps ESCC cells escape from ferroptosis induced by D-lactate, then support ESCC-CSCs’ properties. Error bars represent mean ± SD (n = 3)
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    Image Search Results


    Fig. 8 D-lactate catabolism prevents ESCC CSCs ferroptosis to sustain their stem-like functions. a Cell viability analysis showing the IC50 value of D-lactate in KYSE410 and KYSE450 cells. b MTS measurement for cell viability of KYSE410 and KYSE450 cells treated with 8 mM and 16 mM D-lactate (D) alone or supplementing ZVAD, CQ, FER and NEC simultaneously. c Immunoblotting results suggesting the expression of ferroptosis markers in KYSE410 and KYSE450 cells with 8 mM or 16 mM D-lactate for 72 h. β-actin was used as an internal reference. d Flow cytometry analysis for lipid reactive oxygen species generation in KYSE410 and KYSE450 cells treated with D-lactate (D). e Histogram showing the relative reactive oxygen species production under the action of 8 mM or 16 mM D-lactate in KYSE410 and KYSE450 cells, respectively. f Flow cytometry results indicating the alternative mitochondrial membrane potential induced by 8 mM or 16 mM D-lactate. JC-1 monomers and JC-1 aggregates represent low and high MMP, respectively. g Histogram demonstrating relative mitochondrial membrane potential in KYSE410 and KYSE450 cells treated with D-lactate. h Histogram indicating changed Fe2+ concentration induced by D-lactate in KYSE410 and KYSE450 cells. i Histograms suggesting the changes of intracellular total GSH, reduced GSH and GSSG content in KYSE410 and KYSE450 cells treated with D-lactate. j MTS measurement showing the sensitivity of ESCC cells on Erastin treatment after CDK7-YAP-LDHD axis was up- regulated. k Working model of D-lactate catabolism driven by CDK7-YAP-LDHD axis promotes pyruvate generation and helps ESCC cells escape from ferroptosis induced by D-lactate, then support ESCC-CSCs’ properties. Error bars represent mean ± SD (n = 3)

    Journal: Signal transduction and targeted therapy

    Article Title: CDK7-YAP-LDHD axis promotes D-lactate elimination and ferroptosis defense to support cancer stem cell-like properties.

    doi: 10.1038/s41392-023-01555-9

    Figure Lengend Snippet: Fig. 8 D-lactate catabolism prevents ESCC CSCs ferroptosis to sustain their stem-like functions. a Cell viability analysis showing the IC50 value of D-lactate in KYSE410 and KYSE450 cells. b MTS measurement for cell viability of KYSE410 and KYSE450 cells treated with 8 mM and 16 mM D-lactate (D) alone or supplementing ZVAD, CQ, FER and NEC simultaneously. c Immunoblotting results suggesting the expression of ferroptosis markers in KYSE410 and KYSE450 cells with 8 mM or 16 mM D-lactate for 72 h. β-actin was used as an internal reference. d Flow cytometry analysis for lipid reactive oxygen species generation in KYSE410 and KYSE450 cells treated with D-lactate (D). e Histogram showing the relative reactive oxygen species production under the action of 8 mM or 16 mM D-lactate in KYSE410 and KYSE450 cells, respectively. f Flow cytometry results indicating the alternative mitochondrial membrane potential induced by 8 mM or 16 mM D-lactate. JC-1 monomers and JC-1 aggregates represent low and high MMP, respectively. g Histogram demonstrating relative mitochondrial membrane potential in KYSE410 and KYSE450 cells treated with D-lactate. h Histogram indicating changed Fe2+ concentration induced by D-lactate in KYSE410 and KYSE450 cells. i Histograms suggesting the changes of intracellular total GSH, reduced GSH and GSSG content in KYSE410 and KYSE450 cells treated with D-lactate. j MTS measurement showing the sensitivity of ESCC cells on Erastin treatment after CDK7-YAP-LDHD axis was up- regulated. k Working model of D-lactate catabolism driven by CDK7-YAP-LDHD axis promotes pyruvate generation and helps ESCC cells escape from ferroptosis induced by D-lactate, then support ESCC-CSCs’ properties. Error bars represent mean ± SD (n = 3)

    Article Snippet: Mitochondrial membrane potential assay Lipophilic fluorescent dye JC-1 (MedChemEXpress, HY-K0601) was used to measure the changes of mitochondrial membrane potential.

    Techniques: Western Blot, Expressing, Flow Cytometry, Membrane, Concentration Assay